HPLC – High-Performance Liquid Chromatography
Nova’s chemical metrology product line leverages HPLC – High-Performance Liquid Chromatography, a quantitative analysis method developed around the 1960.
HPLC is a separation technique whereby all relevant components are separated before being measured.
At the beginning of the analysis, a small sample (10-100 µL) is delivered through an HPLC column at the mobile or eluent phase. Due to their interaction with the stationary phase, the sampled solution components pass through the column at different speeds depending on their interaction with the stationary phase. The least interacting components move faster through the column (elute). The stronger the interaction – the longer the retention at the column.
A detector at the end of the column identifies the separated components. The detector’s signal is recorded and used to calculate the concentration of the analyzed components.
Highlights and Benefits
- Fully Automated compact workstation
- Capable of simultaneously determining multiple components in one run
- Leading method, for precision measurement of organic components – even with very low concentration
- Detection of unexpected and undesired components such as contaminants and breakdowns, which accumulate during the lifetime of the plating bath
- Low reagent consumption
- Analyzes clear and turbid solutions
High flexibility due to numerous separation and detection principles